9/15/2023 0 Comments Aging zebrafish circuli![]() ![]() When working on processes that are associated with brain aging, gene expression profiling provides an efficient platform to observe the genetic activity at both an individual, and an organismal level. Future studies will be aimed at determining the causal relationships of age-related changes in gene expression in individual male and female brains, as well as possible interventions that counteract these alterations.īoth genetic and environmental factors contribute to brain aging and there is individual variation in these age-related changes. The gene expression of multiple pathways is altered with age and differentially expressed in males and females. Our results demonstrated that in the brains of young and old male and female zebrafish there were over 500 differentially expressed genes associated with multiple pathways but most notably were those related to neurogenesis and cell differentiation, as well as brain and nervous system development. Statistical analyses were performed using a two-way ANOVA and the relevant post-hoc tests. RNA was isolated from individual brains and subjected to microarray and qPCR analysis. We chose males and females from specific age groups (young, 7.5-8.5 months and old, 31-36 months) based on the progression of cognitive decline in zebrafish. Our aim was to identify the main genetic pathways associated with zebrafish brain aging across gender. Few studies have examined the biological changes in the aging brain that may contribute to these declines and none have examined them within individuals with respect to gender. The zebrafish has recently become a popular model organism for examining aging and age-related diseases because as in humans they age gradually and exhibit cognitive decline. Brain aging is a multi-factorial process due to both genetic and environmental factors. ![]()
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